3DJU

Crystal structure of human BTG2

3DJU の概要

• エントリーDOI: 10.2210/pdb3dju/pdb
• 関連するPDBエントリー: 3DJN
• 分子名称: Protein BTG2 (2 entities in total)
• 機能のキーワード: helix-turn-helix, transcription, transcription regulation
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13767.77

構造登録者

Yang, X.,Morita, M.,Wang, H.,Suzuki, T.,Bartlam, M.,Yamamoto, T. (登録日: 2008-06-24, 公開日: 2008-11-11, 最終更新日: 2023-11-01)

主引用文献

Yang, X.,Morita, M.,Wang, H.,Suzuki, T.,Yang, W.,Luo, Y.,Zhao, C.,Yu, Y.,Bartlam, M.,Yamamoto, T.,Rao, Z.
Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity
Nucleic Acids Res., 36:6872-6881, 2008

PubMed Abstract: BTG2 is the prototypical member of the TOB family and is known to be involved in cell growth, differentiation and DNA repair. As a transcriptional co-regulator, BTG2 interacts with CCR4-associated factor 1 (CAF1) and POP2 (CALIF), which are key components of the general CCR4/NOT multi-subunit transcription complex, and which are reported to play distinct roles as nucleases involved in mRNA deadenylation. Here we report the crystal structures of human BTG2 and mouse TIS21 to 2.3 A and 2.2 A resolution, respectively. The structures reveal the putative CAF1 binding site. CAF1 deadenylase assays were performed with wild-type BTG2 and mutants that disrupt the interaction with CAF1. The results reveal the suppressive role of BTG2 in the regulation of CAF1 deadenylase activity. Our study provides insights into the formation of the BTG2-CAF1 complex and the potential role of BTG2 in the regulation of CAF1.

PubMed: 18974182
DOI: 10.1093/nar/gkn825

実験手法

X-RAY DIFFRACTION (2.26 Å)