3DH7

Structure of T. thermophilus IDI-2 in complex with PPi

3DH7 の概要

• エントリーDOI: 10.2210/pdb3dh7/pdb
• 分子名称: Isopentenyl-diphosphate delta-isomerase, FLAVIN MONONUCLEOTIDE, PYROPHOSPHATE 2-, ... (4 entities in total)
• 機能のキーワード: idi, complex, isomerase, plasmid
• 由来する生物種: Thermus thermophilus HB8
• 細胞内の位置: Cytoplasm (By similarity): Q53W52
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 146444.71

構造登録者

de Ruyck, J.,Wouters, J. (登録日: 2008-06-17, 公開日: 2008-09-23, 最終更新日: 2024-03-20)

主引用文献

de Ruyck, J.,Pouyez, J.,Rothman, S.C.,Poulter, D.,Wouters, J.
Crystal structure of type 2 isopentenyl diphosphate isomerase from Thermus thermophilus in complex with inorganic pyrophosphate
Biochemistry, 47:9051-9053, 2008

PubMed Abstract: The N-terminal region is stabilized in the crystal structure of Thermus thermophilus type 2 isopentenyl diphosphate isomerase in complex with inorganic pyrophosphate, providing new insights about the active site and the catalytic mechanism of the enzyme. The PP i moiety is located near the conserved residues, H10, R97, H152, Q157, E158, and W219, and the flavin cofactor. The putative active site of isopentenyl diphosphate isomerase 2 provides interactions for stabilizing a carbocationic intermediate similar to those that stabilize the intermediate in the well-established protonation-deprotonation mechanism of isopentenyl diphosphate isomerase 1.

PubMed: 18693754
DOI: 10.1021/bi801159x

実験手法

X-RAY DIFFRACTION (2.97 Å)