3DH4

Crystal Structure of Sodium/Sugar symporter with bound Galactose from vibrio parahaemolyticus

3DH4 の概要

• エントリーDOI: 10.2210/pdb3dh4/pdb
• 分子名称: Sodium/glucose cotransporter, beta-D-galactopyranose, ERBIUM (III) ION, ... (4 entities in total)
• 機能のキーワード: membrane protein, symporter, sugar transport, sglt, ion transport, membrane, sodium transport, symport, transmembrane, transport, transport protein
• 由来する生物種: Vibrio parahaemolyticus
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: P96169
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 229217.12

構造登録者

Abramson, J.,Faham, S.,Cascio, D. (登録日: 2008-06-16, 公開日: 2008-08-05, 最終更新日: 2024-02-21)

主引用文献

Faham, S.,Watanabe, A.,Besserer, G.M.,Cascio, D.,Specht, A.,Hirayama, B.A.,Wright, E.M.,Abramson, J.
The crystal structure of a sodium galactose transporter reveals mechanistic insights into Na+/sugar symport.
Science, 321:810-814, 2008

PubMed Abstract: Membrane transporters that use energy stored in sodium gradients to drive nutrients into cells constitute a major class of proteins. We report the crystal structure of a member of the solute sodium symporters (SSS), the Vibrio parahaemolyticus sodium/galactose symporter (vSGLT). The approximately 3.0 angstrom structure contains 14 transmembrane (TM) helices in an inward-facing conformation with a core structure of inverted repeats of 5 TM helices (TM2 to TM6 and TM7 to TM11). Galactose is bound in the center of the core, occluded from the outside solutions by hydrophobic residues. Surprisingly, the architecture of the core is similar to that of the leucine transporter (LeuT) from a different gene family. Modeling the outward-facing conformation based on the LeuT structure, in conjunction with biophysical data, provides insight into structural rearrangements for active transport.

PubMed: 18599740
DOI: 10.1126/science.1160406

実験手法

X-RAY DIFFRACTION (2.7 Å)