3DH2

Crystal structure of ribonuclease Sa2 with guanosine-3'-cyclophosphate prepared by cocrystallization

3DH2 の概要

• エントリーDOI: 10.2210/pdb3dh2/pdb
• 関連するPDBエントリー: 1gmp 1py3 1pyl 3d4a 3d5i 3dgy
• 分子名称: Ribonuclease, beta-D-glucopyranose, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: ribonuclease, hydrolase, protein-mononucleotide complex, guanosine-3'-monophosphate, mononucleotide, cocrystallization
• 由来する生物種: Streptomyces aureofaciens
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45725.46

構造登録者

Sevcik, J.,Bauerova-Hlinkova, V. (登録日: 2008-06-16, 公開日: 2009-06-16, 最終更新日: 2024-11-20)

主引用文献

Bauerova-Hlinkova, V.,Dvorsky, R.,Perecko, D.,Povazanec, F.,Sevcik, J.
Structure of RNase Sa2 complexes with mononucleotides - new aspects of catalytic reaction and substrate recognition
Febs J., 276:4156-4168, 2009

PubMed Abstract: Although the mechanism of RNA cleavage by RNases has been studied for many years, there remain aspects that have not yet been fully clarified. We have solved the crystal structures of RNase Sa2 in the apo form and in complexes with mononucleotides. These structures provide more details about the mechanism of RNA cleavage by RNase Sa2. In addition to Glu56 and His86, which are the principal catalytic residues, an important role in the first reaction step of RNA cleavage also seems to be played by Arg67 and Arg71, which are located in the phosphate-binding site and form hydrogen bonds with the oxygens of the phosphate group of the mononucleotides. Their positive charge very likely causes polarization of the bonds between the oxygens and the phosphorus atom, leading to electron deficiency on the phosphorus atom and facilitating nucleophilic attack by O2' of the ribose on the phosphorus atom, leading to cyclophosphate formation. The negatively charged Glu56 is in position to attract the proton from O2' of the ribose. Extended molecular docking of mononucleotides, dinucleotides and trinucleotides into the active site of the enzyme allowed us to better understand the guanosine specificity of RNase Sa2 and to predict possible binding subsites for the downstream base and ribose of the second and third nucleotides.

PubMed: 19558492
DOI: 10.1111/j.1742-4658.2009.07125.x

実験手法

X-RAY DIFFRACTION (2.25 Å)