3DEP

Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43

3DEP の概要

• エントリーDOI: 10.2210/pdb3dep/pdb
• 関連するPDBエントリー: 3DEO
• 分子名称: Signal recognition particle 43 kDa protein, YPGGSFDPLGLA, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: chloroplast srp system, signal recognition particle, signal sequence, ankyrin repeat, chromodomain, type i turn, substrate protein recognition, l18 region, lhcp, ank repeat, chloroplast, coiled coil, plastid, ribonucleoprotein, protein transport, membrane protein
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress); 詳細
• 細胞内の位置: Plastid, chloroplast stroma: O22265
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21271.31

構造登録者

Holdermann, I.,Stengel, K.F.,Wild, K.,Sinning, I. (登録日: 2008-06-10, 公開日: 2008-08-12, 最終更新日: 2023-11-01)

主引用文献

Stengel, K.F.,Holdermann, I.,Cain, P.,Robinson, C.,Wild, K.,Sinning, I.
Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43.
Science, 321:253-256, 2008

PubMed Abstract: Secretory and membrane proteins carry amino-terminal signal sequences that, in cotranslational targeting, are recognized by the signal recognition particle protein SRP54 without sequence specificity. The most abundant membrane proteins on Earth are the light-harvesting chlorophyll a/b binding proteins (LHCPs). They are synthesized in the cytoplasm, imported into the chloroplast, and posttranslationally targeted to the thylakoid membrane by cpSRP, a heterodimer formed by cpSRP54 and cpSRP43. We present the 1.5 angstrom crystal structure of cpSRP43 characterized by a unique arrangement of chromodomains and ankyrin repeats. The overall shape and charge distribution of cpSRP43 resembles the SRP RNA, which is absent in chloroplasts. The complex with the internal signal sequence of LHCPs reveals that cpSRP43 specifically recognizes a DPLG peptide motif. We describe how cpSPR43 adapts the universally conserved SRP system to posttranslational targeting and insertion of the LHCP family of membrane proteins.

PubMed: 18621669
DOI: 10.1126/science.1158640

実験手法

X-RAY DIFFRACTION (2.7 Å)