3DAW

Structure of the actin-depolymerizing factor homology domain in complex with actin

3DAW の概要

• エントリーDOI: 10.2210/pdb3daw/pdb
• 関連するPDBエントリー: 2A42 2HD7
• 分子名称: Actin, alpha skeletal muscle, Twinfilin-1, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: actin depolymerisation, actin binding proteins, cytoskeleton, structural protein-contractile protein complex, structural protein-structural protein regulator complex, structural protein/structural protein regulator
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: P68135; Cytoplasm: Q91YR1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61995.19

構造登録者

Paavilainen, V.O.,Oksanen, E.,Goldman, A.,Lappalainen, P. (登録日: 2008-05-30, 公開日: 2008-07-29, 最終更新日: 2023-11-01)

主引用文献

Paavilainen, V.O.,Oksanen, E.,Goldman, A.,Lappalainen, P.
Structure of the actin-depolymerizing factor homology domain in complex with actin
J.Cell Biol., 182:51-59, 2008

PubMed Abstract: Actin dynamics provide the driving force for many cellular processes including motility and endocytosis. Among the central cytoskeletal regulators are actin-depolymerizing factor (ADF)/cofilin, which depolymerizes actin filaments, and twinfilin, which sequesters actin monomers and caps filament barbed ends. Both interact with actin through an ADF homology (ADF-H) domain, which is also found in several other actin-binding proteins. However, in the absence of an atomic structure for the ADF-H domain in complex with actin, the mechanism by which these proteins interact with actin has remained unknown. Here, we present the crystal structure of twinfilin's C-terminal ADF-H domain in complex with an actin monomer. This domain binds between actin subdomains 1 and 3 through an interface that is conserved among ADF-H domain proteins. Based on this structure, we suggest a mechanism by which ADF/cofilin and twinfilin inhibit nucleotide exchange of actin monomers and present a model for how ADF/cofilin induces filament depolymerization by weakening intrafilament interactions.

PubMed: 18625842
DOI: 10.1083/jcb.200803100

実験手法

X-RAY DIFFRACTION (2.55 Å)