3D9S

Human Aquaporin 5 (AQP5) - High Resolution X-ray Structure

3D9S の概要

• エントリーDOI: 10.2210/pdb3d9s/pdb
• 分子名称: Aquaporin-5, O-[(S)-{[(2S)-2-(hexanoyloxy)-3-(tetradecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-D-serine (3 entities in total)
• 機能のキーワード: aquaporin, aqp, aquaglyceroporin, membrane protein, water transport, lipid, phosphatidylserine, psf, npa, ar/r, water channel, glycoprotein, membrane, transmembrane, transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P55064
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 114164.23

構造登録者

Horsefield, R.,Norden, K.,Fellert, M.,Backmark, A.,Tornroth-Horsefield, S.,Terwisscha Van Scheltinga, A.C.,Kvassman, J.,Kjellbom, P.,Johanson, U.,Neutze, R. (登録日: 2008-05-27, 公開日: 2008-08-26, 最終更新日: 2023-11-01)

主引用文献

Horsefield, R.,Norden, K.,Fellert, M.,Backmark, A.,Tornroth-Horsefield, S.,Terwisscha van Scheltinga, A.C.,Kvassman, J.,Kjellbom, P.,Johanson, U.,Neutze, R.
High-resolution x-ray structure of human aquaporin 5
Proc.Natl.Acad.Sci.Usa, 105:13327-13332, 2008

PubMed Abstract: Human aquaporin 5 (HsAQP5) facilitates the transport of water across plasma membranes and has been identified within cells of the stomach, duodenum, pancreas, airways, lungs, salivary glands, sweat glands, eyes, lacrimal glands, and the inner ear. AQP5, like AQP2, is subject to posttranslational regulation by phosphorylation, at which point it is trafficked between intracellular storage compartments and the plasma membrane. Details concerning the molecular mechanism of membrane trafficking are unknown. Here we report the x-ray structure of HsAQP5 to 2.0-A resolution and highlight structural similarities and differences relative to other eukaryotic aquaporins. A lipid occludes the putative central pore, preventing the passage of gas or ions through the center of the tetramer. Multiple consensus phosphorylation sites are observed in the structure and their potential regulatory role is discussed. We postulate that a change in the conformation of the C terminus may arise from the phosphorylation of AQP5 and thereby signal trafficking.

PubMed: 18768791
DOI: 10.1073/pnas.0801466105

実験手法

X-RAY DIFFRACTION (2 Å)