3D7W

Mistletoe Lectin I in Complex with Zeatin

「3CEF」から置き換えられました

3D7W の概要

• エントリーDOI: 10.2210/pdb3d7w/pdb
• 関連するPDBエントリー: 1M2T 2R9K
• 分子名称: Beta-galactoside-specific lectin 1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: mistletoe lectin i, cytokinin, zeatin, plant hormones, microgravity, glycoprotein, hydrolase, lectin, plant defense, protein synthesis inhibitor, toxin
• 由来する生物種: Viscum album (European mistletoe); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59027.58

構造登録者

Meyer, A.,Rypniewski, W.,Szymanski, M.,Voelter, W.,Barciszewski, J.,Betzel, C. (登録日: 2008-05-22, 公開日: 2008-06-17, 最終更新日: 2024-10-30)

主引用文献

Meyer, A.,Rypniewski, W.,Szymanski, M.,Voelter, W.,Barciszewski, J.,Betzel, C.
Structure of mistletoe lectin I from Viscum album in complex with the phytohormone zeatin
Biochim.Biophys.Acta, 1784:1590-1595, 2008

PubMed Abstract: The crystal structure of mistletoe lectin I (ML-I) isolated from the European mistletoe Viscum album in complex with the most active phytohormone zeatin has been analyzed and refined to 2.54 A resolution. X-ray suitable crystals of ML-I were obtained by the counter-diffusion method using the Gel-Tube R crystallization kit (GT-R) onboard the Russian Service Module on the international space station ISS. High quality hexagonal bipyramidal crystals were grown during 3 months under microgravity conditions. Selected crystals were soaked in a saturated solution of zeatin and subsequently diffraction data were collected applying synchrotron radiation. A distinct F(o)-F(c) electron density has been found inside a binding pocket located in subunit B of ML-I and has been interpreted as a single zeatin molecule. The structure was refined to investigate the zeatin-ML-I interactions in detail. The results demonstrate the ability of mistletoe to protect itself from the host transpiration regulation by absorbing the most active host plant hormones as part of a defense mechanism.

PubMed: 18718563
DOI: 10.1016/j.bbapap.2008.07.010

実験手法

X-RAY DIFFRACTION (2.49 Å)