3D36

How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the Inhibitor Sda

3D36 の概要

• エントリーDOI: 10.2210/pdb3d36/pdb
• 分子名称: Sporulation kinase B, Sporulation kinase inhibitor Sda, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: ghkl atpase, four helix bundle, class i two-component histidine kinase, kinase, phosphoprotein, transferase, two-component regulatory system, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60894.81

構造登録者

Bick, M.J.,Lamour, V.,Rajashankar, K.R.,Gordiyenko, Y.,Robinson, C.V.,Darst, S.A. (登録日: 2008-05-09, 公開日: 2009-01-13, 最終更新日: 2024-02-21)

主引用文献

Bick, M.J.,Lamour, V.,Rajashankar, K.R.,Gordiyenko, Y.,Robinson, C.V.,Darst, S.A.
How to switch off a histidine kinase: crystal structure of Geobacillus stearothermophilus KinB with the inhibitor Sda
J.Mol.Biol., 386:163-177, 2009

PubMed Abstract: Entry to sporulation in bacilli is governed by a histidine kinase phosphorelay, a variation of the predominant signal transduction mechanism in prokaryotes. Sda directly inhibits sporulation histidine kinases in response to DNA damage and replication defects. We determined a 2.0-A-resolution X-ray crystal structure of the intact cytoplasmic catalytic core [comprising the dimerization and histidine phosphotransfer domain (DHp domain), connected to the ATP binding catalytic domain] of the Geobacillus stearothermophilus sporulation kinase KinB complexed with Sda. Structural and biochemical analyses reveal that Sda binds to the base of the DHp domain and prevents molecular transactions with the DHp domain to which it is bound by acting as a simple molecular barricade. Sda acts to sterically block communication between the catalytic domain and the DHp domain, which is required for autophosphorylation, as well as to sterically block communication between the response regulator Spo0F and the DHp domain, which is required for phosphotransfer and phosphatase activities.

PubMed: 19101565
DOI: 10.1016/j.jmb.2008.12.006

実験手法

X-RAY DIFFRACTION (2.03 Å)