3D1N

Structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone gene promoter

3D1N の概要

• エントリーDOI: 10.2210/pdb3d1n/pdb
• 関連するPDBエントリー: 1au7 1oct
• 分子名称: 5'-D(*DAP*DGP*DCP*DAP*DTP*DAP*DAP*DAP*DTP*DAP*DAP*DTP*DAP*DA)-3', 5'-D(*DTP*DTP*DAP*DTP*DTP*DAP*DTP*DTP*DTP*DAP*DTP*DGP*DCP*DT)-3', POU domain, class 6, transcription factor 1, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, helix-turn-helix (hth), dna-binding, homeobox, nucleus, transcription, transcription regulation, transcription regulator-dna complex, transcription regulator/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus: 3D1N
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 175754.48

構造登録者

Pereira, J.H.,Ha, S.C.,Kim, S.-H. (登録日: 2008-05-06, 公開日: 2009-05-26, 最終更新日: 2024-10-30)

主引用文献

Pereira, J.H.,Kim, S.H.
Structure of human Brn-5 transcription factor in complex with CRH gene promoter.
J.Struct.Biol., 167:159-165, 2009

PubMed Abstract: The Brn-5 protein, highly expressed in human brain, belongs to the POU family; a class of transcription factors involved in a wide variety of biological processes ranging from programming of embryonic stem cells to cellular housekeeping. This functional diversity is conferred by two DNA-binding subdomains that can assume several configurations due to a bipartite arrangement of POU-specific (POU(S)) and POU-homeo (POU(H)) subdomains separated by a linker region. The crystal structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone (CRH) gene promoter reveals an unexpected recognition mode of the protein to its cognate DNA. Moreover, the structure also shows the role of the linker in allowing diverse configurations that can be assumed by the two subdomains.

PubMed: 19450691
DOI: 10.1016/j.jsb.2009.05.003

実験手法

X-RAY DIFFRACTION (2.51 Å)