3D0U

Crystal Structure of Lysine Riboswitch Bound to Lysine

3D0U の概要

• エントリーDOI: 10.2210/pdb3d0u/pdb
• 分子名称: Lysine Riboswitch RNA, IRIDIUM HEXAMMINE ION, LYSINE, ... (4 entities in total)
• 機能のキーワード: rna-ligand complex, riboswitch, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53495.68

構造登録者

Garst, A.D.,Heroux, A.,Rambo, R.P.,Batey, R.T. (登録日: 2008-05-02, 公開日: 2008-07-01, 最終更新日: 2024-02-21)

主引用文献

Garst, A.D.,Heroux, A.,Rambo, R.P.,Batey, R.T.
Crystal structure of the lysine riboswitch regulatory mRNA element.
J.Biol.Chem., 283:22347-22351, 2008

PubMed Abstract: Riboswitches are metabolite-sensitive elements found in mRNAs that control gene expression through a regulatory secondary structural switch. Along with regulation of lysine biosynthetic genes, mutations within the lysine-responsive riboswitch (L-box) play a role in the acquisition of resistance to antimicrobial lysine analogs. To understand the structural basis for lysine binding, we have determined the 2.8 angstroms resolution crystal structure of lysine bound to the Thermotoga maritima asd lysine riboswitch ligand-binding domain. The structure reveals a complex architecture scaffolding a binding pocket completely enveloping lysine. Mutations conferring antimicrobial resistance cluster around this site as well as highly conserved long range interactions, indicating that they disrupt lysine binding or proper folding of the RNA. Comparison of the free and bound forms by x-ray crystallography, small angle x-ray scattering, and chemical probing reveals almost identical structures, indicating that lysine induces only limited and local conformational changes upon binding.

PubMed: 18593706
DOI: 10.1074/jbc.C800120200

実験手法

X-RAY DIFFRACTION (2.8 Å)