3CXH

Structure of yeast complex III with isoform-2 cytochrome c bound and definition of a minimal core interface for electron transfer.

3CXH の概要

• エントリーDOI: 10.2210/pdb3cxh/pdb
• 関連するPDBエントリー: 1EZV 1KB9 1KYO 3CX5
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, HEAVY CHAIN (VH) OF FV-FRAGMENT, LIGHT CHAIN (VL) OF FV-FRAGMENT, ... (23 entities in total)
• 機能のキーワード: complex iii, cytochrome c isoform-2, electron transfer complex, cytochrome bc1 complex, mitochondrialtransmembrane complex, respiratory chain, transient protein-protein interaction, electron transport, inner membrane, mitochondrion, transit peptide, transport, phosphoprotein, heme, iron, metal-binding, iron-sulfur, oxidoreductase
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 533742.33

構造登録者

Solmaz, S.R.N.,Hunte, C. (登録日: 2008-04-24, 公開日: 2008-05-13, 最終更新日: 2023-08-30)

主引用文献

Solmaz, S.R.,Hunte, C.
Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer.
J.Biol.Chem., 283:17542-17549, 2008

PubMed Abstract: In cellular respiration, cytochrome c transfers electrons from cytochrome bc(1) complex (complex III) to cytochrome c oxidase by transiently binding to the membrane proteins. Here, we report the structure of isoform-1 cytochrome c bound to cytochrome bc(1) complex at 1.9 A resolution in reduced state. The dimer structure is asymmetric. Monovalent cytochrome c binding is correlated with conformational changes of the Rieske head domain and subunit QCR6p and with a higher number of interfacial water molecules bound to cytochrome c(1). Pronounced hydration and a "mobility mismatch" at the interface with disordered charged residues on the cytochrome c side are favorable for transient binding. Within the hydrophobic interface, a minimal core was identified by comparison with the novel structure of the complex with bound isoform-2 cytochrome c. Four core interactions encircle the heme cofactors surrounded by variable interactions. The core interface may be a feature to gain specificity for formation of the reactive complex.

PubMed: 18390544
DOI: 10.1074/jbc.M710126200

実験手法

X-RAY DIFFRACTION (2.5 Å)