3CW5

E. coli Initiator tRNA

3CW5 の概要

• エントリーDOI: 10.2210/pdb3cw5/pdb
• 関連するPDBエントリー: 3CW6
• 分子名称: Initiator tRNA (1 entity in total)
• 機能のキーワード: trna, initiator, rna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24832.92

構造登録者

Barraud, P.,Schmitt, E.,Mechulam, Y.,Dardel, F.,Tisne, C. (登録日: 2008-04-21, 公開日: 2008-09-02, 最終更新日: 2023-08-30)

主引用文献

Barraud, P.,Schmitt, E.,Mechulam, Y.,Dardel, F.,Tisne, C.
A unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis.
Nucleic Acids Res., 36:4894-4901, 2008

PubMed Abstract: In all organisms, translational initiation takes place on the small ribosomal subunit and two classes of methionine tRNA are present. The initiator is used exclusively for initiation of protein synthesis while the elongator is used for inserting methionine internally in the nascent polypeptide chain. The crystal structure of Escherichia coli initiator tRNA(f)(Met) has been solved at 3.1 A resolution. The anticodon region is well-defined and reveals a unique structure, which has not been described in any other tRNA. It encompasses a Cm32*A38 base pair with a peculiar geometry extending the anticodon helix, a base triple between A37 and the G29-C41 pair in the major groove of the anticodon stem and a modified stacking organization of the anticodon loop. This conformation is associated with the three GC basepairs in the anticodon stem, characteristic of initiator tRNAs and suggests a mechanism by which the translation initiation machinery could discriminate the initiator tRNA from all other tRNAs.

PubMed: 18653533
DOI: 10.1093/nar/gkn462

実験手法

X-RAY DIFFRACTION (3.1 Å)