3CVF

Crystal Structure of the carboxy terminus of Homer3

3CVF の概要

• エントリーDOI: 10.2210/pdb3cvf/pdb
• 関連するPDBエントリー: 3CVE
• 分子名称: Homer protein homolog 3 (2 entities in total)
• 機能のキーワード: coiled coil, alternative splicing, cell junction, cytoplasm, membrane, phosphoprotein, polymorphism, postsynaptic cell membrane, synapse, signaling protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm : Q9NSC5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 36250.88

構造登録者

Hayashi, M.K.,Stearns, M.H.,Giannini, V.,Xu, R.-M.,Sala, C.,Hayashi, Y. (登録日: 2008-04-18, 公開日: 2009-03-31, 最終更新日: 2024-11-06)

主引用文献

Hayashi, M.K.,Tang, C.,Verpelli, C.,Narayanan, R.,Stearns, M.H.,Xu, R.M.,Li, H.,Sala, C.,Hayashi, Y.
The postsynaptic density proteins Homer and Shank form a polymeric network structure.
Cell(Cambridge,Mass.), 137:159-171, 2009

PubMed Abstract: The postsynaptic density (PSD) is crucial for synaptic functions, but the molecular architecture retaining its structure and components remains elusive. Homer and Shank are among the most abundant scaffolding proteins in the PSD, working synergistically for maturation of dendritic spines. Here, we demonstrate that Homer and Shank, together, form a mesh-like matrix structure. Crystallographic analysis of this region revealed a pair of parallel dimeric coiled coils intercalated in a tail-to-tail fashion to form a tetramer, giving rise to the unique configuration of a pair of N-terminal EVH1 domains at each end of the coiled coil. In neurons, the tetramerization is required for structural integrity of the dendritic spines and recruitment of proteins to synapses. We propose that the Homer-Shank complex serves as a structural framework and as an assembly platform for other PSD proteins.

PubMed: 19345194
DOI: 10.1016/j.cell.2009.01.050

実験手法

X-RAY DIFFRACTION (2.9 Å)