3CTT

Crystal complex of N-terminal Human Maltase-Glucoamylase with Casuarine

3CTT の概要

• エントリーDOI: 10.2210/pdb3ctt/pdb
• 関連するPDBエントリー: 2qly 2qmj
• 分子名称: Maltase-glucoamylase, 2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: glycosyl hydrolase family 31, alpha-glucosidase, glycoprotein, glycosidase, membrane, multifunctional enzyme, signal-anchor, sulfation, transmembrane, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Apical cell membrane; Single-pass type II membrane protein: O43451
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 100158.52

構造登録者

Sim, L.,Rose, D.R. (登録日: 2008-04-14, 公開日: 2009-02-24, 最終更新日: 2024-10-30)

主引用文献

Cardona, F.,Parmeggiani, C.,Faggi, E.,Bonaccini, C.,Gratteri, P.,Sim, L.,Gloster, T.M.,Roberts, S.,Davies, G.J.,Rose, D.R.,Goti, A.
Total syntheses of casuarine and its 6-O-alpha-glucoside: complementary inhibition towards glycoside hydrolases of the GH31 and GH37 families
Chemistry, 15:1627-1636, 2009

PubMed Abstract: Total synthesis of naturally occurring casuarine (1) and the first total synthesis of casuarine 6-O-alpha-glucoside (2) were achieved through complete stereoselective nitrone cycloaddition, Tamao-Fleming oxidation and selective alpha-glucosylation as key steps. Biological assays of the two compounds proved their strong and selective inhibitory properties towards glucoamylase NtMGAM and trehalase Tre37A, respectively, which place them among the most powerful inhibitors of these enzymes. The structural determination of the complexes of NtMGAM with 1 and of Tre37A with 2 revealed interesting similarities in the catalytic sites of these two enzymes which belong to different families and clans.

PubMed: 19123216
DOI: 10.1002/chem.200801578

実験手法

X-RAY DIFFRACTION (2.1 Å)