Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3CRX

CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I

Summary for 3CRX
Entry DOI10.2210/pdb3crx/pdb
DescriptorDNA 35-MER, CRE RECOMBINASE, ... (6 entities in total)
Functional Keywordscre recombinase, holliday junction, recombination, recombinase-dna complex, hydrolase, ligase-dna complex, ligase/dna
Biological sourceEnterobacteria phage P1
Total number of polymer chains6
Total formula weight120170.22
Authors
Gopaul, D.N.,Guo, F.,Vanduyne, G.D. (deposition date: 1998-06-19, release date: 1999-12-14, Last modification date: 2023-08-02)
Primary citationGopaul, D.N.,Guo, F.,Van Duyne, G.D.
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination.
EMBO J., 17:4175-4187, 1998
Cited by
PubMed Abstract: We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.
PubMed: 9670032
DOI: 10.1093/emboj/17.14.4175
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.5 Å)
Structure validation

226707

数据于2024-10-30公开中

PDB statisticsPDBj update infoContact PDBjnumon