3CRX

CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I

3CRX の概要

• エントリーDOI: 10.2210/pdb3crx/pdb
• 分子名称: DNA 35-MER, CRE RECOMBINASE, ... (6 entities in total)
• 機能のキーワード: cre recombinase, holliday junction, recombination, recombinase-dna complex, hydrolase, ligase-dna complex, ligase/dna
• 由来する生物種: Enterobacteria phage P1
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 120170.22

構造登録者

Gopaul, D.N.,Guo, F.,Vanduyne, G.D. (登録日: 1998-06-19, 公開日: 1999-12-14, 最終更新日: 2023-08-02)

主引用文献

Gopaul, D.N.,Guo, F.,Van Duyne, G.D.
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination.
EMBO J., 17:4175-4187, 1998

PubMed Abstract: We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.

PubMed: 9670032
DOI: 10.1093/emboj/17.14.4175

実験手法

X-RAY DIFFRACTION (2.5 Å)