3CRT

Structural characterization of an engineered allosteric protein

Summary for 3CRT

Related1GNE 3CRS 3CRU
DescriptorGlutathione S-transferase class-mu 26 kDa isozyme, GLUTATHIONE (3 entities in total)
Functional Keywordsprotein design, engineered allostery. ph-switch, transferase
Biological sourceSchistosoma japonicum (Blood fluke)
Total number of polymer chains1
Total molecular weight25370.47
Authors
Sagermann, M.,Chapleau, R.,DeLorimier, E.,Lei, M. (deposition date: 2008-04-07, release date: 2009-02-24, Last modification date: 2011-07-13)
Primary citation
Sagermann, M.,Chapleau, R.R.,DeLorimier, E.,Lei, M.
Using affinity chromatography to engineer and characterize pH-dependent protein switches.
Protein Sci., 18:217-228, 2009
PubMed: 19177365 (PDB entries with the same primary citation)
DOI: 10.1002/pro.23
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.9 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.266280.9%8.4%7.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution