3COQ

Structural Basis for Dimerization in DNA Recognition by Gal4

3COQ の概要

• エントリーDOI: 10.2210/pdb3coq/pdb
• 分子名称: DNA (5'-D(*DAP*DCP*DCP*DGP*DGP*DAP*DGP*DGP*DAP*DCP*DAP*DGP*DTP*DCP*DCP*DTP*DCP*DCP*DGP*DG)-3'), DNA (5'-D(*DTP*DCP*DCP*DGP*DGP*DAP*DGP*DGP*DAP*DCP*DTP*DGP*DTP*DCP*DCP*DTP*DCP*DCP*DGP*DG)-3'), Regulatory protein GAL4, ... (6 entities in total)
• 機能のキーワード: helix bundle, protein-dna complex, zinc binuclear cluster, activator, carbohydrate metabolism, dna-binding, galactose metabolism, metal-binding, nucleus, phosphoprotein, transcription, transcription regulation, transcription-dna complex, transcription/dna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 33620.73

構造登録者

Hong, M.,Fitzgerald, M.X.,Harper, S.,Luo, C.,Speicher, D.W. (登録日: 2008-03-29, 公開日: 2008-07-01, 最終更新日: 2024-02-21)

主引用文献

Hong, M.,Fitzgerald, M.X.,Harper, S.,Luo, C.,Speicher, D.W.,Marmorstein, R.
Structural basis for dimerization in DNA recognition by gal4.
Structure, 16:1019-1026, 2008

PubMed Abstract: Gal4 is a Zn2Cys6 binuclear cluster containing transcription factor that binds DNA as a homodimer and can activate transcription by interacting with the mutant Gal11P protein. Although structures have been reported of the Gal4 dimerization domain and the binuclear cluster domain bound to DNA as a dimer, the structure of the "complete" Gal4 dimer bound to DNA has not previously been described. Here we report the structure of a complete Gal4 dimer bound to DNA and additional biochemical studies to address the molecular basis for Gal4 dimerization in DNA binding. We find that Gal4 dimerization on DNA is mediated by an intertwined helical bundle that deviates significantly from the solution NMR structure of the free dimerization domain. Associated biochemical studies show that the dimerization domain of Gal4 is important for DNA binding and protein thermostability. We also map the interaction surface of the Gal4 dimerization domain with Gal11P.

PubMed: 18611375
DOI: 10.1016/j.str.2008.03.015

実験手法

X-RAY DIFFRACTION (2.4 Å)