3CO1

Crystal structure of microtubule binding domain of human EB3

3CO1 の概要

• エントリーDOI: 10.2210/pdb3co1/pdb
• 関連するPDBエントリー: 1PA7 1WYO
• 分子名称: Microtubule-associated protein RP/EB family member 3 (2 entities in total)
• 機能のキーワード: rp/eb family, ch domain, microtubule-binding, +tip protein, cell cycle, cell division, mitosis, phosphoprotein, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton: Q9UPY8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15402.82

構造登録者

De Groot, C.O.,Honnappa, S.,Steinmetz, M.O. (登録日: 2008-03-27, 公開日: 2009-03-17, 最終更新日: 2023-11-01)

主引用文献

Komarova, Y.,De Groot, C.O.,Grigoriev, I.,Gouveia, S.M.,Munteanu, E.L.,Schober, J.M.,Honnappa, S.,Buey, R.M.,Hoogenraad, C.C.,Dogterom, M.,Borisy, G.G.,Steinmetz, M.O.,Akhmanova, A.
Mammalian end binding proteins control persistent microtubule growth.
J.Cell Biol., 2009

PubMed Abstract: End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles of the three mammalian EBs in controlling microtubule dynamics and analyzed the domains involved. Protein depletion and rescue experiments showed that EB1 and EB3, but not EB2, promote persistent microtubule growth by suppressing catastrophes. Furthermore, we demonstrated in vitro and in cells that the EB plus-end tracking behavior depends on the calponin homology domain but does not require dimer formation. In contrast, dimerization is necessary for the EB anti-catastrophe activity in cells; this explains why the EB1 dimerization domain, which disrupts native EB dimers, exhibits a dominant-negative effect. When microtubule dynamics is reconstituted with purified tubulin, EBs promote rather than inhibit catastrophes, suggesting that in cells EBs prevent catastrophes by counteracting other microtubule regulators. This probably occurs through their action on microtubule ends, because catastrophe suppression does not require the EB domains needed for binding to known EB partners.

PubMed: 19255245
DOI: 10.1083/jcb.200807179

実験手法

X-RAY DIFFRACTION (1.4 Å)