3CMS

ENGINEERING ENZYME SUB-SITE SPECIFICITY: PREPARATION, KINETIC CHARACTERIZATION AND X-RAY ANALYSIS AT 2.0-ANGSTROMS RESOLUTION OF VAL111PHE SITE-MUTATED CALF CHYMOSIN

3CMS の概要

• エントリーDOI: 10.2210/pdb3cms/pdb
• 分子名称: CHYMOSIN B (2 entities in total)
• 機能のキーワード: hydrolase, acid proteinase
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35720.74

構造登録者

Newman, M.,Frazao, C.,Shearer, A.,Tickle, I.J.,Blundell, T.L. (登録日: 1990-02-26, 公開日: 1992-10-15, 最終更新日: 2017-11-29)

• 主引用文献: Strop, P.,Sedlacek, J.,Stys, J.,Kaderabkova, Z.,Blaha, I.,Pavlickova, L.,Pohl, J.,Fabry, M.,Kostka, V.,Newman, M.; Engineering enzyme subsite specificity: preparation, kinetic characterization, and X-ray analysis at 2.0-A resolution of Val111Phe site-mutated calf chymosin.; Biochemistry, 29:9863-9871, 1990; PubMed: 2271625; DOI: 10.1021/bi00494a016

実験手法

X-RAY DIFFRACTION (2 Å)