3CL3

Crystal Structure of a vFLIP-IKKgamma complex: Insights into viral activation of the IKK signalosome

3CL3 の概要

• エントリーDOI: 10.2210/pdb3cl3/pdb
• 分子名称: ORF K13, NF-kappa-B essential modulator (2 entities in total)
• 機能のキーワード: death effector domain, coiled-coil, coiled coil, cytoplasm, disease mutation, ectodermal dysplasia, host-virus interaction, nucleus, transcription, transcription regulation, viral protein-signaling protein complex, viral protein/signaling protein
• 由来する生物種: Human gammaherpesvirus 8; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 70846.87

構造登録者

Bagneris, C.,Ageichik, A.V.,Cronin, N.,Boshoff, C.,Waksman, G.,Barrett, T. (登録日: 2008-03-18, 公開日: 2008-06-17, 最終更新日: 2024-02-21)

主引用文献

Bagneris, C.,Ageichik, A.V.,Cronin, N.,Wallace, B.,Collins, M.,Boshoff, C.,Waksman, G.,Barrett, T.
Crystal structure of a vFlip-IKKgamma complex: insights into viral activation of the IKK signalosome.
Mol.Cell, 30:620-631, 2008

PubMed Abstract: Key to the pathogenicity of several viruses is activation of the canonical nuclear factor-kappaB (NF-kappaB) transcriptional pathway. Subversion of this tightly regulated mechanism is achieved through the production of host mimetic viral proteins that deregulate the transcription process. One such protein is ks-vFLIP (produced by the Kaposi's sarcoma herpes virus [KSHV]), which associates with IKKgamma, an essential component of the IKK complex or signalosome. This interaction renders the canonical NF-kappaB pathway constitutively active and has been linked to Kaposi's sarcoma and other malignancies. In order to elucidate the molecular basis underpinning ks-vFLIP-induced activation of the IKK signalosome, we have determined the crystal structure of a complex involving a fragment of IKKgamma bound to ks-vFLIP at 3.2 A. In addition to identifying and subsequently probing the ks-vFLIP-IKKgamma interface, we have also investigated the effects of a mutation implicated in the genetic disorder anhydrotic ectodermal dysplasia with immunodeficiency (EDA-ID).

PubMed: 18538660
DOI: 10.1016/j.molcel.2008.04.029

実験手法

X-RAY DIFFRACTION (3.2 Å)