3CI0
The Crystal Structure of the GspK-GspI-GspJ complex from enterotoxigenic Escherichia coli Type 2 Secretion System
Summary for 3CI0
| Entry DOI | 10.2210/pdb3ci0/pdb |
| Descriptor | Pseudopilin GspI, Pseudopilin GspJ, Pseudopilin GspK, ... (6 entities in total) |
| Functional Keywords | general secretory pathway, pseudopilus, type 4 pilin biogenesis, methylation, protein transport |
| Biological source | Escherichia coli More |
| Cellular location | Cell inner membrane (By similarity): A7ZRI8 |
| Total number of polymer chains | 3 |
| Total formula weight | 62640.15 |
| Authors | Korotkov, K.V.,Hol, W.G.J. (deposition date: 2008-03-10, release date: 2008-04-29, Last modification date: 2024-10-16) |
| Primary citation | Korotkov, K.V.,Hol, W.G. Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system. Nat.Struct.Mol.Biol., 15:462-468, 2008 Cited by PubMed Abstract: Gram-negative bacteria translocate various proteins including virulence factors across their outer membrane via type 2 secretion systems (T2SSs). T2SSs are thought to contain a pseudopilus, a subcomplex formed by one major and several minor pseudopilins. We report the crystal structure of the complex formed by three minor pseudopilins from enterotoxigenic Escherichia coli. The GspK-GspI-GspJ complex has quasihelical characteristics and an architecture consistent with a localization at the pseudopilus tip. The alpha-domain of GspK has a previously unobserved fold with an unexpected dinuclear metal binding site. The area surrounding its disulfide bridge is conserved and might interact with other T2SS components or with secreted proteins. PubMed: 18438417DOI: 10.1038/nsmb.1426 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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