3CI0

The Crystal Structure of the GspK-GspI-GspJ complex from enterotoxigenic Escherichia coli Type 2 Secretion System

3CI0 の概要

• エントリーDOI: 10.2210/pdb3ci0/pdb
• 分子名称: Pseudopilin GspI, Pseudopilin GspJ, Pseudopilin GspK, ... (6 entities in total)
• 機能のキーワード: general secretory pathway, pseudopilus, type 4 pilin biogenesis, methylation, protein transport
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell inner membrane (By similarity): A7ZRI8
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62640.15

構造登録者

Korotkov, K.V.,Hol, W.G.J. (登録日: 2008-03-10, 公開日: 2008-04-29, 最終更新日: 2024-10-16)

主引用文献

Korotkov, K.V.,Hol, W.G.
Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system.
Nat.Struct.Mol.Biol., 15:462-468, 2008

PubMed Abstract: Gram-negative bacteria translocate various proteins including virulence factors across their outer membrane via type 2 secretion systems (T2SSs). T2SSs are thought to contain a pseudopilus, a subcomplex formed by one major and several minor pseudopilins. We report the crystal structure of the complex formed by three minor pseudopilins from enterotoxigenic Escherichia coli. The GspK-GspI-GspJ complex has quasihelical characteristics and an architecture consistent with a localization at the pseudopilus tip. The alpha-domain of GspK has a previously unobserved fold with an unexpected dinuclear metal binding site. The area surrounding its disulfide bridge is conserved and might interact with other T2SS components or with secreted proteins.

PubMed: 18438417
DOI: 10.1038/nsmb.1426

実験手法

X-RAY DIFFRACTION (2.2 Å)