3CH2

Crystal Structure Analysis of SERA5E from plasmodium falciparum

3CH2 の概要

• エントリーDOI: 10.2210/pdb3ch2/pdb
• 関連するPDBエントリー: 3CH3
• 分子名称: Serine-repeat antigen protein, CALCIUM ION (3 entities in total)
• 機能のキーワード: cysteine protease, papain family, glycoprotein, hydrolase, malaria, thiol protease, vacuole
• 由来する生物種: Plasmodium falciparum
• 細胞内の位置: Parasitophorous vacuole : Q9TY95
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30704.32

構造登録者

Smith, B.J.,Malby, R.L.,Colman, P.M.,Clarke, O.B. (登録日: 2008-03-07, 公開日: 2008-03-25, 最終更新日: 2024-10-16)

主引用文献

Hodder, A.N.,Malby, R.L.,Clarke, O.B.,Fairlie, W.D.,Colman, P.M.,Crabb, B.S.,Smith, B.J.
Structural Insights into the Protease-like Antigen Plasmodium falciparum SERA5 and Its Noncanonical Active-Site Serine
J.Mol.Biol., 392:154-165, 2009

PubMed Abstract: The sera genes of the malaria-causing parasite Plasmodium encode a family of unique proteins that are maximally expressed at the time of egress of parasites from infected red blood cells. These multi-domain proteins are unique, containing a central papain-like cysteine-protease fragment enclosed between the disulfide-linked N- and C-terminal domains. However, the central fragment of several members of this family, including serine repeat antigen 5 (SERA5), contains a serine (S596) in place of the active-site cysteine. Here we report the crystal structure of the central protease-like domain of Plasmodium falciparum SERA5, revealing a number of anomalies in addition to the putative nucleophilic serine: (1) the structure of the putative active site is not conducive to binding substrate in the canonical cysteine-protease manner; (2) the side chain of D594 restricts access of substrate to the putative active site; and (3) the S(2) specificity pocket is occupied by the side chain of Y735, reducing this site to a small depression on the protein surface. Attempts to determine the structure in complex with known inhibitors were not successful. Thus, despite having revealed its structure, the function of the catalytic domain of SERA5 remains an enigma.

PubMed: 19591843
DOI: 10.1016/j.jmb.2009.07.007

実験手法

X-RAY DIFFRACTION (1.8 Å)