3CG3

Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate

3CG3 の概要

• エントリーDOI: 10.2210/pdb3cg3/pdb
• 関連するPDBエントリー: 2onr 3CFX 3CG1 3CG3
• 分子名称: UPF0100 protein PH0151, TUNGSTATE(VI)ION (3 entities in total)
• 機能のキーワード: abc transporter, binding protein, molybdate, tungstate, ligand, unknown function, transport protein
• 由来する生物種: Pyrococcus horikoshii
• 細胞内の位置: Cell membrane; Peripheral membrane protein (By similarity): O57890
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36685.81

構造登録者

Comellas-Bigler, M.,Hollenstein, K.,Locher, K.P. (登録日: 2008-03-04, 公開日: 2009-03-10, 最終更新日: 2024-02-21)

主引用文献

Hollenstein, K.,Comellas-Bigler, M.,Bevers, L.E.,Feiters, M.C.,Meyer-Klaucke, W.,Hagedoorn, P.L.,Locher, K.P.
Distorted octahedral coordination of tungstate in a subfamily of specific binding proteins.
J.Biol.Inorg.Chem., 14:663-672, 2009

PubMed Abstract: Bacteria and archaea import molybdenum and tungsten from the environment in the form of the oxyanions molybdate (MoO(4) (2-)) and tungstate (WO(4) (2-)). These substrates are captured by an external, high-affinity binding protein, and delivered to ATP binding cassette transporters, which move them across the cell membrane. We have recently reported a crystal structure of the molybdate/tungstate binding protein ModA/WtpA from Archaeoglobus fulgidus, which revealed an octahedrally coordinated central metal atom. By contrast, the previously determined structures of three bacterial homologs showed tetracoordinate molybdenum and tungsten atoms in their binding pockets. Until then, coordination numbers above four had only been found for molybdenum/tungsten in metalloenzymes where these metal atoms are part of the catalytic cofactors and coordinated by mostly non-oxygen ligands. We now report a high-resolution structure of A. fulgidus ModA/WtpA, as well as crystal structures of four additional homologs, all bound to tungstate. These crystal structures match X-ray absorption spectroscopy measurements from soluble, tungstate-bound protein, and reveal the details of the distorted octahedral coordination. Our results demonstrate that the distorted octahedral geometry is not an exclusive feature of the A. fulgidus protein, and suggest distinct binding modes of the binding proteins from archaea and bacteria.

PubMed: 19234723
DOI: 10.1007/s00775-009-0479-7

実験手法

X-RAY DIFFRACTION (1.8 Å)