3CFE
Crystal structure of purple-fluorescent antibody EP2-25C10
Summary for 3CFE
| Entry DOI | 10.2210/pdb3cfe/pdb |
| Related | 1FL3 1UB5 1UB6 3CFB 3CFC 3CFD |
| Descriptor | PURPLE-FLUORESCENT ANTIBODY EP2-25C10-KAPPA LIGHT CHAIN, PURPLE-FLUORESCENT ANTIBODY EP2-25C10-IGG2B HEAVY CHAIN, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | immunoglobulin, purple-fluorescent antibody, hapten complex, immune system |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 4 |
| Total formula weight | 95579.14 |
| Authors | Debler, E.W.,Heine, A.,Wilson, I.A. (deposition date: 2008-03-03, release date: 2008-03-18, Last modification date: 2024-11-13) |
| Primary citation | Debler, E.W.,Kaufmann, G.F.,Meijler, M.M.,Heine, A.,Mee, J.M.,Pljevaljcic, G.,Di Bilio, A.J.,Schultz, P.G.,Millar, D.P.,Janda, K.D.,Wilson, I.A.,Gray, H.B.,Lerner, R.A. Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex. Science, 319:1232-1235, 2008 Cited by PubMed Abstract: The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody. PubMed: 18309081DOI: 10.1126/science.1153445 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.99 Å) |
Structure validation
Download full validation report
