3CFC

High-resolution structure of blue fluorescent antibody EP2-19G2

3CFC の概要

• エントリーDOI: 10.2210/pdb3cfc/pdb
• 関連するPDBエントリー: 1FL3 1UB5 1UB6 3CFB 3CFD 3CFE
• 分子名称: BLUE FLUORESCENT ANTIBODY EP2-19G2-IGG2B HEAVY CHAIN, BLUE FLUORESCENT ANTIBODY EP2-19G2-KAPPA LIGHT CHAIN, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: immunoglobulin, blue-fluorescent antibody, hapten complex, immune system, electron transfer
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46921.53

構造登録者

Debler, E.W.,Wilson, I.A. (登録日: 2008-03-03, 公開日: 2008-03-18, 最終更新日: 2024-10-16)

主引用文献

Debler, E.W.,Kaufmann, G.F.,Meijler, M.M.,Heine, A.,Mee, J.M.,Pljevaljcic, G.,Di Bilio, A.J.,Schultz, P.G.,Millar, D.P.,Janda, K.D.,Wilson, I.A.,Gray, H.B.,Lerner, R.A.
Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.
Science, 319:1232-1235, 2008

PubMed Abstract: The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

PubMed: 18309081
DOI: 10.1126/science.1153445

実験手法

X-RAY DIFFRACTION (1.7 Å)