3CFB

High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K

Summary for 3CFB

• Entry DOI: 10.2210/pdb3cfb/pdb
• Related: 1FL3 1UB5 1UB6 3CFC 3CFD 3CFE
• Descriptor: BLUE FLUORESCENT ANTIBODY EP2-19G2-KAPPA LIGHT CHAIN, BLUE FLUORESCENT ANTIBODY EP2-19G2-IGG2B HEAVY CHAIN, 4-(4-STYRYL-PHENYLCARBAMOYL)-BUTYRIC ACID, ... (5 entities in total)
• Functional Keywords: immunoglobulin, blue-fluorescent antibody, hapten complex, immune system, electron transfer
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 94832.13

Authors

Debler, E.W.,Wilson, I.A. (deposition date: 2008-03-03, release date: 2008-03-18, Last modification date: 2024-10-30)

Primary citation

Debler, E.W.,Kaufmann, G.F.,Meijler, M.M.,Heine, A.,Mee, J.M.,Pljevaljcic, G.,Di Bilio, A.J.,Schultz, P.G.,Millar, D.P.,Janda, K.D.,Wilson, I.A.,Gray, H.B.,Lerner, R.A.
Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.
Science, 319:1232-1235, 2008

PubMed Abstract: The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

PubMed: 18309081
DOI: 10.1126/science.1153445

Experimental method

X-RAY DIFFRACTION (1.6 Å)