3CEG

Crystal structure of the UBC domain of baculoviral IAP repeat-containing protein 6

3CEG の概要

• エントリーDOI: 10.2210/pdb3ceg/pdb
• 分子名称: Baculoviral IAP repeat-containing protein 6 (2 entities in total)
• 機能のキーワード: apoptosis, ligase, protease inhibitor, thiol protease inhibitor, ubl conjugation pathway, structural genomics consortium, sgc
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane: Q9NR09
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74111.32

構造登録者

Walker, J.R.,Avvakumov, G.V.,Xue, S.,Butler-Cole, C.,Bountra, C.,Weigelt, J.,Arrowsmith, C.H.,Edwards, A.M.,Bochkarev, A.,Dhe-Paganon, S.,Structural Genomics Consortium (SGC) (登録日: 2008-02-29, 公開日: 2008-04-01, 最終更新日: 2024-10-30)

主引用文献

Sheng, Y.,Hong, J.H.,Doherty, R.,Srikumar, T.,Shloush, J.,Avvakumov, G.V.,Walker, J.R.,Xue, S.,Neculai, D.,Wan, J.W.,Kim, S.K.,Arrowsmith, C.H.,Raught, B.,Dhe-Paganon, S.
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Mol Cell Proteomics, 11:329-341, 2012

PubMed Abstract: Here we describe a systematic structure-function analysis of the human ubiquitin (Ub) E2 conjugating proteins, consisting of the determination of 15 new high-resolution three-dimensional structures of E2 catalytic domains, and autoubiquitylation assays for 26 Ub-loading E2s screened against a panel of nine different HECT (homologous to E6-AP carboxyl terminus) E3 ligase domains. Integration of our structural and biochemical data revealed several E2 surface properties associated with Ub chain building activity; (1) net positive or neutral E2 charge, (2) an "acidic trough" located near the catalytic Cys, surrounded by an extensive basic region, and (3) similarity to the previously described HECT binding signature in UBE2L3 (UbcH7). Mass spectrometry was used to characterize the autoubiquitylation products of a number of functional E2-HECT pairs, and demonstrated that HECT domains from different subfamilies catalyze the formation of very different types of Ub chains, largely independent of the E2 in the reaction. Our data set represents the first comprehensive analysis of E2-HECT E3 interactions, and thus provides a framework for better understanding the molecular mechanisms of ubiquitylation.

PubMed: 22496338
DOI: 10.1074/mcp.O111.013706

実験手法

X-RAY DIFFRACTION (2.008 Å)