3CA9

Evolution of chlorella virus dUTPase

Summary for 3CA9

• Entry DOI: 10.2210/pdb3ca9/pdb
• Related: 3C2T 3C3I
• Descriptor: Deoxyuridine triphosphatase, MAGNESIUM ION, DEOXYURIDINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: dutpase chlorella virus, hydrolase
• Biological source: Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)
• Total number of polymer chains: 2
• Total formula weight: 35904.74

Authors

Yamanishi, M.,Homma, K.,Zhang, Y.,Etten, L.V.J.,Moriyama, H. (deposition date: 2008-02-19, release date: 2009-03-03, Last modification date: 2024-02-21)

Primary citation

Homma, K.,Moriyama, H.
Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase.
Acta Crystallogr.,Sect.F, 65:1030-1034, 2009

PubMed Abstract: The 141-amino-acid deoxyuridine triphosphatase (dUTPase) from the algal Chlorella virus IL-3A and its Glu81Ser/Thr84Arg-mutant derivative Mu-22 were crystallized using the hanging-drop vapor-diffusion method at 298 K with polyethylene glycol as the precipitant. An apo IL-3A dUTPase with an amino-terminal T7 epitope tag and a carboxy-terminal histidine tag yielded cubic P2(1)3 crystals with unit-cell parameter a = 106.65 A. In the presence of dUDP, the enzyme produced thin stacked orthorhombic P222 crystals with unit-cell parameters a = 81.0, b = 96.2, c = 132.8 A. T7-histidine-tagged Mu-22 dUTPase formed thin stacked rectangular crystals. Amino-terminal histidine-tagged dUTPases did not crystallize but formed aggregates. Glycyl-seryl-tagged dUTPases yielded cubic P2(1)3 IL-3A crystals with unit-cell parameter a = 105.68 A and hexagonal P6(3) Mu-22 crystals with unit-cell parameters a = 132.07, c = 53.45 A, gamma = 120 degrees . Owing to the Thr84Arg mutation, Mu-22 dUTPase had different monomer-to-monomer interactions to those of IL-3A dUTPase.

PubMed: 19851015
DOI: 10.1107/S1744309109034459

Experimental method

X-RAY DIFFRACTION (3 Å)