3C7N

Structure of the Hsp110:Hsc70 Nucleotide Exchange Complex

3C7N の概要

• エントリーDOI: 10.2210/pdb3c7n/pdb
• 関連するPDBエントリー: 1YUW 2QXL
• 分子名称: Heat shock protein homolog SSE1, Heat shock cognate, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: chaperone, hsp110, hsp70, hsc70, molecular chaperone, atp state, acetylation, atp-binding, adp, calmodulin binding, cytoplasm, mucleotide binding, phosphorylation, stress response, calmodulin-binding, nucleotide-binding, phosphoprotein, nucleus, transcription, chaperone-chaperone complex, chaperone/chaperone
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm : P32589 P19120
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 137082.59

構造登録者

Schuermann, J.P.,Jiang, J.,Hart, P.J.,Sousa, R. (登録日: 2008-02-07, 公開日: 2008-05-27, 最終更新日: 2024-02-21)

主引用文献

Schuermann, J.P.,Jiang, J.,Cuellar, J.,Llorca, O.,Wang, L.,Gimenez, L.E.,Jin, S.,Taylor, A.B.,Demeler, B.,Morano, K.A.,Hart, P.J.,Valpuesta, J.M.,Lafer, E.M.,Sousa, R.
Structure of the Hsp110:Hsc70 nucleotide exchange machine
Mol.Cell, 31:232-243, 2008

PubMed Abstract: Hsp70s mediate protein folding, translocation, and macromolecular complex remodeling reactions. Their activities are regulated by proteins that exchange ADP for ATP from the nucleotide-binding domain (NBD) of the Hsp70. These nucleotide exchange factors (NEFs) include the Hsp110s, which are themselves members of the Hsp70 family. We report the structure of an Hsp110:Hsc70 nucleotide exchange complex. The complex is characterized by extensive protein:protein interactions and symmetric bridging interactions between the nucleotides bound in each partner protein's NBD. An electropositive pore allows nucleotides to enter and exit the complex. The role of nucleotides in complex formation and dissociation, and the effects of the protein:protein interactions on nucleotide exchange, can be understood in terms of the coupled effects of the nucleotides and protein:protein interactions on the open-closed isomerization of the NBDs. The symmetrical interactions in the complex may model other Hsp70 family heterodimers in which two Hsp70s reciprocally act as NEFs.

PubMed: 18550409
DOI: 10.1016/j.molcel.2008.05.006

実験手法

X-RAY DIFFRACTION (3.115 Å)