3C0T

Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18

Summary for 3C0T

• Entry DOI: 10.2210/pdb3c0t/pdb
• Related: 2HZM 2HZS
• Descriptor: Mediator of RNA polymerase II transcription subunit 18, Mediator of RNA polymerase II transcription subunit 8 (3 entities in total)
• Functional Keywords: beta barrel, channel, protein-protein complex, activator, nucleus, transcription, transcription regulation
• Biological source: Schizosaccharomyces pombe (fission yeast); More
• Cellular location: Nucleus (By similarity): O14198; Nucleus (Probable): O94646
• Total number of polymer chains: 2
• Total formula weight: 27915.73

Authors

Lariviere, L.,Seizl, M.,van Wageningen, S.,Roether, S.,van de Pasch, L.,Feldmann, H.,Straesser, K.,Hahn, S.,Holstege, C.P.,Cramer, P. (deposition date: 2008-01-21, release date: 2008-04-22, Last modification date: 2024-04-03)

Primary citation

Lariviere, L.,Seizl, M.,van Wageningen, S.,Rother, S.,van de Pasch, L.,Feldmann, H.,Strasser, K.,Hahn, S.,Holstege, F.C.P.,Cramer, P.
Structure-system correlation identifies a gene regulatory Mediator submodule
Genes Dev., 22:872-877, 2008

PubMed Abstract: A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.

PubMed: 18381891
DOI: 10.1101/gad.465108

Experimental method

X-RAY DIFFRACTION (2.4 Å)