3C0T

Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18

3C0T の概要

• エントリーDOI: 10.2210/pdb3c0t/pdb
• 関連するPDBエントリー: 2HZM 2HZS
• 分子名称: Mediator of RNA polymerase II transcription subunit 18, Mediator of RNA polymerase II transcription subunit 8 (3 entities in total)
• 機能のキーワード: beta barrel, channel, protein-protein complex, activator, nucleus, transcription, transcription regulation
• 由来する生物種: Schizosaccharomyces pombe (fission yeast); 詳細
• 細胞内の位置: Nucleus (By similarity): O14198; Nucleus (Probable): O94646
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27915.73

構造登録者

Lariviere, L.,Seizl, M.,van Wageningen, S.,Roether, S.,van de Pasch, L.,Feldmann, H.,Straesser, K.,Hahn, S.,Holstege, C.P.,Cramer, P. (登録日: 2008-01-21, 公開日: 2008-04-22, 最終更新日: 2024-04-03)

主引用文献

Lariviere, L.,Seizl, M.,van Wageningen, S.,Rother, S.,van de Pasch, L.,Feldmann, H.,Strasser, K.,Hahn, S.,Holstege, F.C.P.,Cramer, P.
Structure-system correlation identifies a gene regulatory Mediator submodule
Genes Dev., 22:872-877, 2008

PubMed Abstract: A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.

PubMed: 18381891
DOI: 10.1101/gad.465108

実験手法

X-RAY DIFFRACTION (2.4 Å)