3BZM

Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF

3BZM の概要

• エントリーDOI: 10.2210/pdb3bzm/pdb
• 関連するPDBエントリー: 3BZN
• 分子名称: Menaquinone-specific isochorismate synthase, CITRIC ACID (3 entities in total)
• 機能のキーワード: chorismate, isochorismate, menaquinone, isomerase, menaquinone biosynthesis
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49015.55

構造登録者

Parsons, J.F.,Shi, K.M.,Ladner, J.E. (登録日: 2008-01-18, 公開日: 2008-05-20, 最終更新日: 2024-02-21)

主引用文献

Parsons, J.F.,Shi, K.M.,Ladner, J.E.
Structure of isochorismate synthase in complex with magnesium.
Acta Crystallogr.,Sect.D, 64:607-610, 2008

PubMed Abstract: The electron carrier menaquinone is one of many important bacterial metabolites that are derived from the key intermediate chorismic acid. MenF, the first enzyme in the menaquinone pathway, catalyzes the isomerization of chorismate to isochorismate. Here, an improved structure of MenF in a new crystal form is presented. The structure, solved at 2.0 angstroms resolution in complex with magnesium, reveals a well defined closed active site. Existing evidence suggests that the mechanism of the reaction catalyzed by MenF involves nucleophilic attack of a water molecule on the chorismate ring. The structure reveals a well defined water molecule located in an appropriate position for activation by Lys190 and attack on the substrate.

PubMed: 18453696
DOI: 10.1107/S0907444908005477

実験手法

X-RAY DIFFRACTION (1.95 Å)