3BY8

Crystal Structure of the E.coli DcuS Sensor Domain

3BY8 の概要

• エントリーDOI: 10.2210/pdb3by8/pdb
• 関連するPDBエントリー: 3BY9
• 分子名称: Sensor protein dcuS, (2S)-2-hydroxybutanedioic acid (3 entities in total)
• 機能のキーワード: histidine kinase sensor domain, inner membrane, membrane, phosphoprotein, transferase, transmembrane, two-component regulatory system
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P0AEC8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15891.92

構造登録者

Cheung, J.,Hendrickson, W.A. (登録日: 2008-01-15, 公開日: 2008-08-12, 最終更新日: 2024-02-21)

主引用文献

Cheung, J.,Hendrickson, W.A.
Crystal Structures of C4-Dicarboxylate Ligand Complexes with Sensor Domains of Histidine Kinases DcuS and DctB.
J.Biol.Chem., 283:30256-30265, 2008

PubMed Abstract: Two-component signaling systems allow bacteria to adapt to changing environments. Typically, a chemical or other stimulus is detected by the periplasmic sensor domain of a transmembrane histidine kinase sensor, which in turn relays a signal through a phosphotransfer cascade to the cognate cytoplasmic response regulator. Such systems lead ultimately to changes in gene expression or cell motility. Mechanisms of ligand binding and signal transduction through the cell membrane in histidine kinases are not fully understood. In an effort to further understand such processes, we have solved the crystal structures of the periplasmic sensor domains of Escherichia coli DcuS and of Vibrio cholerae DctB in complex with the respective cognate ligands, malate and succinate. Both proteins are involved in the regulation of the transport and metabolism of C(4)-dicarboxylates, but they are not highly related by sequence similarity. Our work reveals that despite disparate sizes, both structures contain a similar characteristic alpha/beta PDC (PhoQ-DcuS-CitA) sensor-domain fold and display similar modes of ligand binding, suggesting similar mechanisms of function.

PubMed: 18701447
DOI: 10.1074/jbc.M805253200

実験手法

X-RAY DIFFRACTION (1.45 Å)