3BW2

Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

3BW2 の概要

• エントリーDOI: 10.2210/pdb3bw2/pdb
• 関連するPDBエントリー: 3BW3 3BW4
• 分子名称: 2-nitropropane dioxygenase, FLAVIN MONONUCLEOTIDE, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: tim barrel, oxidoreductase, dioxygenase
• 由来する生物種: Streptomyces ansochromogenes
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39118.88

構造登録者

Li, Y.H.,Gao, Z.Q.,Hou, H.F. (登録日: 2008-01-08, 公開日: 2009-01-13, 最終更新日: 2024-03-13)

主引用文献

Li, Y.H.,Gao, Z.Q.,Hou, H.F.,Li, L.,Zhang, J.H.,Yang, H.H.,Dong, Y.H.,Tan, H.R.
Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes
Biochem.Biophys.Res.Commun., 405:344-348, 2011

PubMed Abstract: Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His179, which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure.

PubMed: 21147069
DOI: 10.1016/j.bbrc.2010.12.050

実験手法

X-RAY DIFFRACTION (2.1 Å)