3BSX

Crystal Structure of Human Pumilio 1 in complex with Puf5 RNA

3BSX の概要

• エントリーDOI: 10.2210/pdb3bsx/pdb
• 関連するPDBエントリー: 1M8W 1M8X 1M8Y 3BSB
• 分子名称: 5'-R(*UP*UP*GP*UP*AP*AP*UP*AP*UP*UP*A)-3', Pumilio homolog 1 (3 entities in total)
• 機能のキーワード: protein-rna complex, phosphoprotein, rna-binding, translation regulation, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm (Probable): Q14671
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 86526.02

構造登録者

Gupta, Y.K.,Nair, D.T.,Wharton, R.P.,Aggarwal, A.K. (登録日: 2007-12-26, 公開日: 2008-04-08, 最終更新日: 2023-11-01)

主引用文献

Gupta, Y.K.,Nair, D.T.,Wharton, R.P.,Aggarwal, A.K.
Structures of human Pumilio with noncognate RNAs reveal molecular mechanisms for binding promiscuity.
Structure, 16:549-557, 2008

PubMed Abstract: Pumilio is a founder member of the evolutionarily conserved Puf family of RNA-binding proteins that control a number of physiological processes in eukaryotes. A structure of human Pumilio (hPum) Puf domain bound to a Drosophila regulatory sequence showed that each Puf repeat recognizes a single nucleotide. Puf domains in general bind promiscuously to a large set of degenerate sequences, but the structural basis for this promiscuity has been unclear. Here, we describe the structures of hPum Puf domain complexed to two noncognate RNAs, CycB(reverse) and Puf5. In each complex, one of the nucleotides is ejected from the binding surface, in effect, acting as a "spacer." The complexes also reveal the plasticity of several Puf repeats, which recognize noncanonical nucleotides. Together, these complexes provide a molecular basis for recognition of degenerate binding sites, which significantly increases the number of mRNAs targeted for regulation by Puf proteins in vivo.

PubMed: 18328718
DOI: 10.1016/j.str.2008.01.006

実験手法

X-RAY DIFFRACTION (2.32 Å)