3BR9

Crystal Structure of HCV NS5B Polymerase with a Novel Pyridazinone Inhibitor

3BR9 の概要

• エントリーDOI: 10.2210/pdb3br9/pdb
• 分子名称: RNA-directed RNA polymerase, (2R)-2-({3-[5-hydroxy-2-(3-methylbutyl)-3-oxo-6-thiophen-2-yl-2,3-dihydropyridazin-4-yl]-1,1-dioxido-2H-1,2,4-benzothia diazin-7-yl}oxy)propanamide (3 entities in total)
• 機能のキーワード: protein-ligand complex, metal-binding, nucleotide-binding, nucleotidyltransferase, ribonucleoprotein, rna replication, rna-binding, rna-directed rna polymerase, transcription, transcription regulation, transferase, cytoplasm, host-virus interaction, antiviral, viral nucleoprotein, hydrolase
• 由来する生物種: Hepatitis C virus
• 細胞内の位置: Core protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein. Core protein p19: Virion (By similarity). Envelope glycoprotein E1: Virion membrane; Single-pass type I membrane protein (Potential). Envelope glycoprotein E2: Virion membrane; Single-pass type I membrane protein (Potential). p7: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Protease NS2-3: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). RNA-directed RNA polymerase: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential): P26663
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 129754.68

構造登録者

Zhao, Q.,Showalter, R.E.,Han, Q.,Kissinger, C.R. (登録日: 2007-12-21, 公開日: 2008-12-23, 最終更新日: 2024-04-03)

• 主引用文献: Zhou, Y.,Webber, S.E.,Murphy, D.E.,Li, L.S.,Dragovich, P.S.,Tran, C.V.,Sun, Z.,Ruebsam, F.,Shah, A.M.,Tsan, M.,Showalter, R.E.,Patel, R.,Li, B.,Zhao, Q.,Han, Q.,Hermann, T.,Kissinger, C.R.,Lebrun, L.,Sergeeva, M.V.,Kirkovsky, L.; Novel HCV NS5B polymerase inhibitors derived from 4-(1',1'-dioxo-1',4'-dihydro-1'lambda6-benzo[1',2',4']thiadiazin-3'-yl)-5-hydroxy-2H-pyridazin-3-ones. Part 1: exploration of 7'-substitution of benzothiadiazine.; Bioorg.Med.Chem.Lett., 18:1413-1418, 2008; PubMed: 18242088; DOI: 10.1016/j.bmcl.2008.01.007

実験手法

X-RAY DIFFRACTION (2.3 Å)