3BK6

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii

3BK6 の概要

• エントリーDOI: 10.2210/pdb3bk6/pdb
• 分子名称: PH stomatin (1 entity in total)
• 機能のキーワード: stomatin, archaea, pyrococcus horikoshii, trimer, coiled-coil, flotillin, spfh, membrane fusion, trafficking, transmembrane, membrane protein
• 由来する生物種: Pyrococcus horikoshii
• 細胞内の位置: Membrane; Single-pass membrane protein (Potential): O59180
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 63892.19

構造登録者

Yokoyama, H.,Fujii, S.,Matsui, I. (登録日: 2007-12-05, 公開日: 2008-02-19, 最終更新日: 2024-03-13)

主引用文献

Yokoyama, H.,Fujii, S.,Matsui, I.
Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold
J.Mol.Biol., 376:868-878, 2008

PubMed Abstract: Stomatin is a major integral membrane protein of human erythrocytes, the absence of which is associated with a form of hemolytic anemia known as hereditary stomatocytosis. However, the function of stomatin is not fully understood. An open reading frame, PH1511, from the hyperthermophilic archaeon Pyrococcus horikoshii encodes p-stomatin, a prokaryotic stomatin. Here, we report the first crystal structure of a stomatin ortholog, the core domain of the p-stomatin PH1511p (residues 56-234 of PH1511p, designated as PhSto(CD)). PhSto(CD) forms a novel homotrimeric structure. Three alpha/beta domains form a triangle of about 50 A on each side, and three alpha-helical segments of about 60 A in length extend from the apexes of the triangle. The alpha/beta domain of PhSto(CD) is partly similar in structure to the band-7 domain of mouse flotillin-2. While the alpha/beta domain is relatively rigid, the alpha-helical segment shows conformational flexibility, adapting to the neighboring environment. One alpha-helical segment forms an anti-parallel coiled coil with another alpha-helical segment from a symmetry-related molecule. The alpha-helical segment shows a heptad repeat pattern, and mainly hydrophobic residues form a coiled-coil interface. According to chemical cross-linking experiments, PhSto(CD) would be able to assemble into an oligomeric form. The coiled-coil fold observed in the crystal probably contributes to self-association.

PubMed: 18182167
DOI: 10.1016/j.jmb.2007.12.024

実験手法

X-RAY DIFFRACTION (3.2 Å)