3BEP

Structure of a sliding clamp on DNA

3BEP の概要

• エントリーDOI: 10.2210/pdb3bep/pdb
• 関連するPDBエントリー: 2POL
• 分子名称: DNA (5'-D(P*DCP*DCP*DCP*DAP*DTP*DCP*DGP*DTP*DAP*DT)-3'), DNA (5'-D(*DTP*DTP*DTP*DTP*DAP*DTP*DAP*DCP*DGP*DAP*DTP*DGP*DGP*DG)-3'), DNA polymerase III subunit beta, ... (5 entities in total)
• 機能のキーワード: beta subunit, sliding clamp, e. coli polymerase iii, dna complex, transferase, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm : P0A988
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 89038.46

構造登録者

Georgescu, R.E.,Kim, S.S.,Yurieva, O.,Kuriyan, J.,Kong, X.-P.,O'Donnell, M. (登録日: 2007-11-19, 公開日: 2008-01-29, 最終更新日: 2023-08-30)

主引用文献

Georgescu, R.E.,Kim, S.S.,Yurieva, O.,Kuriyan, J.,Kong, X.-P.,O'Donnell, M.
Structure of a sliding clamp on DNA
Cell(Cambridge,Mass.), 132:43-54, 2008

PubMed Abstract: The structure of the E. coli beta clamp polymerase processivity factor has been solved in complex with primed DNA. Interestingly, the clamp directly binds the DNA duplex and also forms a crystal contact with the ssDNA template strand, which binds into the protein-binding pocket of the clamp. We demonstrate that these clamp-DNA interactions function in clamp loading, perhaps by inducing the ring to close around DNA. Clamp binding to template ssDNA may also serve to hold the clamp at a primed site after loading or during switching of multiple factors on the clamp. Remarkably, the DNA is highly tilted as it passes through the beta ring. The pronounced 22 degrees angle of DNA through beta may enable DNA to switch between multiple factors bound to a single clamp simply by alternating from one protomer of the ring to the other.

PubMed: 18191219
DOI: 10.1016/j.cell.2007.11.045

実験手法

X-RAY DIFFRACTION (1.92 Å)