3BDF

Crystal structure of metal-free E. coli alkaline phosphatase (T155V)

Summary for 3BDF

• Entry DOI: 10.2210/pdb3bdf/pdb
• Related: 3BDG 3BDH
• Descriptor: Alkaline phosphatase, SULFATE ION (3 entities in total)
• Functional Keywords: bacterial alkaline phosphatase, hydrolase, magnesium, metal-binding, periplasm, phosphorylation, zinc
• Biological source: Escherichia coli
• Cellular location: Periplasm: P00634
• Total number of polymer chains: 2
• Total formula weight: 97025.77

Authors

Grigg, J.C.,Murphy, M.E. (deposition date: 2007-11-14, release date: 2008-11-25, Last modification date: 2024-10-30)

• Primary citation: Grigg, J.C.,Hucaluk, C.,Murphy, M.E.,Ritter, H.,Yee, J.,Rafferty, S.; The Active-Site Trimetallic Cluster of Alkaline Phosphatase is Lost Upon Isosteric Mutation at the Mg2+-Coordinating Residue Threonine-155; To be Published,

Experimental method

X-RAY DIFFRACTION (1.4 Å)