3B8N

Structure of FepE- Bacterial Polysaccharide Co-polymerase

Summary for 3B8N

• Entry DOI: 10.2210/pdb3b8n/pdb
• Related: 3B8M 3B8O 3B8P
• Descriptor: Ferric enterobactin (Enterochelin) transport (1 entity in total)
• Functional Keywords: wzz, fepe, bacterial polysaccharide co-polymerase, metal transport, biosynthetic protein
• Biological source: Escherichia coli
• Total number of polymer chains: 9
• Total formula weight: 285623.17

Authors

Tocilj, A.,Matte, A.,Cygler, M. (deposition date: 2007-11-01, release date: 2008-01-22, Last modification date: 2024-02-21)

Primary citation

Tocilj, A.,Munger, C.,Proteau, A.,Morona, R.,Purins, L.,Ajamian, E.,Wagner, J.,Papadopoulos, M.,Van Den Bosch, L.,Rubinstein, J.L.,Fethiere, J.,Matte, A.,Cygler, M.
Bacterial polysaccharide co-polymerases share a common framework for control of polymer length
Nat.Struct.Mol.Biol., 15:130-138, 2008

PubMed Abstract: The chain length distribution of complex polysaccharides present on the bacterial surface is determined by polysaccharide co-polymerases (PCPs) anchored in the inner membrane. We report crystal structures of the periplasmic domains of three PCPs that impart substantially different chain length distributions to surface polysaccharides. Despite very low sequence similarities, they have a common protomer structure with a long central alpha-helix extending 100 A into the periplasm. The protomers self-assemble into bell-shaped oligomers of variable sizes, with a large internal cavity. Electron microscopy shows that one of the full-length PCPs has a similar organization as that observed in the crystal for its periplasmic domain alone. Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery.

PubMed: 18204465
DOI: 10.1038/nsmb.1374

Experimental method

X-RAY DIFFRACTION (3.1 Å)