3B4A

T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate

3B4A の概要

• エントリーDOI: 10.2210/pdb3b4a/pdb
• 関連するPDBエントリー: 2ho7 2z74 2z75 3B4B 3B4C
• 分子名称: glmS ribozyme substrate RNA, glmS ribozyme RNA, 2-amino-2-deoxy-6-O-phosphono-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: ribozyme, riboswitch, glucosamine-6-phosphate, rna
• 由来する生物種: Thermoanaerobacter tengcongensis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49925.43

構造登録者

Klein, D.J.,Ferre-D'Amare, A.R. (登録日: 2007-10-23, 公開日: 2007-12-11, 最終更新日: 2024-02-21)

主引用文献

Klein, D.J.,Been, M.D.,Ferre-D'Amare, A.R.
Essential Role of an Active-Site Guanine in glmS Ribozyme Catalysis.
J.Am.Chem.Soc., 129:14858-14859, 2007

PubMed Abstract: The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.

PubMed: 17990888
DOI: 10.1021/ja0768441

実験手法

X-RAY DIFFRACTION (2.7 Å)