3B0I

Crystal structure of recombinant human alpha lactalbumin

3B0I の概要

• エントリーDOI: 10.2210/pdb3b0i/pdb
• 関連するPDBエントリー: 3B0K 3B0O
• 分子名称: Alpha-lactalbumin, CALCIUM ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: calcium binding protein, glycoprotein, metal binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P00709
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14360.47

構造登録者

Makabe, K. (登録日: 2011-06-10, 公開日: 2012-06-13, 最終更新日: 2024-11-13)

主引用文献

Makabe, K.,Nakamura, T.,Kuwajima, K.
Structural insights into the stability perturbations induced by N-terminal variation in human and goat alpha-lactalbumin
Protein Eng.Des.Sel., 26:165-170, 2013

PubMed Abstract: Addition of an extra methionine at the N-terminus by recombinant expression of α-lactalbumin in Escherichia coli significantly destabilizes the protein, and this destabilization has hampered mutational analyses such as the mutational phi-value analysis of the protein. Deletion of residue 1 from the recombinant form recovers the stability in human and goat α-lactalbumin. Here, we thus determined the crystal structures of the residue 1-deletion variants of recombinant human and goat α-lactalbumin, and compared the structures with those of the authentic and recombinant forms. The results demonstrate the importance of the N-terminal backbone structure and hydrogen-bonding pattern for the stability of α-lactalbumin.

PubMed: 23155056
DOI: 10.1093/protein/gzs093

実験手法

X-RAY DIFFRACTION (1.8 Å)