3AZC

Crystal structure of the soluble part of cytochrome b6f complex iron-sulfur subunit from Thermosynechococcus elongatus BP-1

Summary for 3AZC

• Entry DOI: 10.2210/pdb3azc/pdb
• Descriptor: Cytochrome b6-f complex iron-sulfur subunit, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• Functional Keywords: rieske, cytochrome b6f complex, thermosynechococcus elongatus, photosynthesis, electron transport, thylakoid membrane, oxidoreductase
• Biological source: Thermosynechococcus elongatus
• Cellular location: Cellular thylakoid membrane; Single-pass membrane protein (By similarity): P0C8N8
• Total number of polymer chains: 1
• Total formula weight: 14821.38

Authors

Veit, S.,Takeda, K.,Tsunoyama, Y.,Roegner, M.,Miki, K. (deposition date: 2011-05-23, release date: 2012-05-23, Last modification date: 2024-11-20)

Primary citation

Veit, S.,Takeda, K.,Tsunoyama, Y.,Rexroth, D.,Rogner, M.,Miki, K.
Structure of a thermophilic cyanobacterial b(6)f-type Rieske protein
Acta Crystallogr.,Sect.D, 68:1400-1408, 2012

PubMed Abstract: The `Rieske protein' PetC is one of the key subunits of the cytochrome b(6)f complex. Its Rieske-type [2Fe-2S] cluster participates in the photosynthetic electron-transport chain. Overexpression and careful structure analysis at 2.0 Å resolution of the extrinsic soluble domain of PetC from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 enabled in-depth spectroscopic and structural characterization and suggested novel structural features. In particular, both the protein structure and the positions of the internal water molecules unexpectedly showed a higher similarity to eukaryotic PetCs than to other prokaryotic PetCs. The structure also revealed a deep pocket on the PetC surface which is oriented towards the membrane surface in the whole complex. Its surface properties suggest a binding site for a hydrophobic compound and the complete conservation of the pocket-forming residues in all known PetC sequences indicates the functional importance of this pocket in the cytochrome b(6)f complex.

PubMed: 22993094
DOI: 10.1107/S0907444912034129

Experimental method

X-RAY DIFFRACTION (2 Å)