3ARD

Ternary crystal structure of the mouse NKT TCR-CD1d-3'deoxy-alpha-galactosylceramide

3ARD の概要

• エントリーDOI: 10.2210/pdb3ard/pdb
• 関連するPDBエントリー: 3ARB 3ARE 3ARF 3ARG 3HE6
• 分子名称: Antigen-presenting glycoprotein CD1d1, Beta-2-microglobulin, NKT Valpha14-Jalpha18,NKT Valpha14-Jalpha18, ... (7 entities in total)
• 機能のキーワード: mouse cd1d, mouse nkt tcr, immune system
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98368.01

構造登録者

Wun, K.S.,Rossjohn, J. (登録日: 2010-11-27, 公開日: 2011-03-30, 最終更新日: 2024-11-13)

主引用文献

Wun, K.S.,Cameron, G.,Patel, O.,Pang, S.S.,Pellicci, D.G.,Sullivan, L.C.,Keshipeddy, S.,Young, M.H.,Uldrich, A.P.,Thakur, M.S.,Richardson, S.K.,Howell, A.R.,Illarionov, P.A.,Brooks, A.G.,Besra, G.S.,McCluskey, J.,Gapin, L.,Porcelli, S.A.,Godfrey, D.I.,Rossjohn, J.
A molecular basis for the exquisite CD1d-restricted antigen specificity and functional responses of natural killer T cells
Immunity, 34:327-339, 2011

PubMed Abstract: Natural killer T (NKT) cells respond to a variety of CD1d-restricted antigens (Ags), although the basis for Ag discrimination by the NKT cell receptor (TCR) is unclear. Here we have described NKT TCR fine specificity against several closely related Ags, termed altered glycolipid ligands (AGLs), which differentially stimulate NKT cells. The structures of five ternary complexes all revealed similar docking. Acyl chain modifications did not affect the interaction, but reduced NKT cell proliferation, indicating an affect on Ag processing or presentation. Conversely, truncation of the phytosphingosine chain caused an induced fit mode of TCR binding that affected TCR affinity. Modifications in the glycosyl head group had a direct impact on the TCR interaction and associated cellular response, with ligand potency reflecting the t(1/2) life of the interaction. Accordingly, we have provided a molecular basis for understanding how modifications in AGLs can result in striking alterations in the cellular response of NKT cells.

PubMed: 21376639
DOI: 10.1016/j.immuni.2011.02.001

実験手法

X-RAY DIFFRACTION (3.01 Å)