3AQ3

Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b

3AQ3 の概要

• エントリーDOI: 10.2210/pdb3aq3/pdb
• 関連するPDBエントリー: 3AQ2 3AQ4
• 分子名称: 6b protein (2 entities in total)
• 機能のキーワード: agrobacterium 6b, toxin, adp-ribosylation, mirna machineries
• 由来する生物種: Agrobacterium tumefaciens
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 73603.93

構造登録者

Wang, M.M.,Yuan, Y.A. (登録日: 2010-10-25, 公開日: 2011-01-26, 最終更新日: 2024-10-30)

主引用文献

Wang, M.M.,Soyano, T.,Machida, S.,Yang, J.Y.,Jung, C.,Chua, N.H.,Yuan, Y.A.
Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b
Genes Dev., 25:64-76, 2011

PubMed Abstract: The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 Å, Agrobacterium vitis AB6b at 1.65 Å, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 Å. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity.

PubMed: 21156810
DOI: 10.1101/gad.1985511

実験手法

X-RAY DIFFRACTION (2.1 Å)