3AP3

Crystal structure of human tyrosylprotein sulfotransferase-2 complexed with PAP

Summary for 3AP3

• Entry DOI: 10.2210/pdb3ap3/pdb
• Related: 3AP1 3AP2
• Descriptor: Protein-tyrosine sulfotransferase 2, ADENOSINE-3'-5'-DIPHOSPHATE (2 entities in total)
• Functional Keywords: sulfotransferase fold, transferase
• Biological source: Homo sapiens (human)
• Cellular location: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity): O60704
• Total number of polymer chains: 4
• Total formula weight: 160263.56

Authors

Teramoto, T.,Fujikawa, Y.,Kawaguchi, Y.,Kurogi, K.,Soejima, M.,Adachi, R.,Nakanishi, Y.,Mishiro-Sato, E.,Liu, M.-C.,Sakakibara, Y.,Suiko, M.,Kimura, M.,Kakuta, Y. (deposition date: 2010-10-09, release date: 2011-10-26, Last modification date: 2024-10-23)

Primary citation

Teramoto, T.,Fujikawa, Y.,Kawaguchi, Y.,Kurogi, K.,Soejima, M.,Adachi, R.,Nakanishi, Y.,Mishiro-Sato, E.,Liu, M.C.,Sakakibara, Y.,Suiko, M.,Kimura, M.,Kakuta, Y.
Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction.
Nat Commun, 4:1572-1572, 2013

PubMed Abstract: Post-translational protein modification by tyrosine sulfation has an important role in extracellular protein-protein interactions. The protein tyrosine sulfation reaction is catalysed by the Golgi enzyme called the tyrosylprotein sulfotransferase. To date, no crystal structure is available for tyrosylprotein sulfotransferase. Detailed mechanism of protein tyrosine sulfation reaction has thus remained unclear. Here we present the first crystal structure of the human tyrosylprotein sulfotransferase isoform 2 complexed with a substrate peptide (C4P5Y3) derived from complement C4 and 3'-phosphoadenosine-5'-phosphate at 1.9 Å resolution. Structural and complementary mutational analyses revealed the molecular basis for catalysis being an SN2-like in-line displacement mechanism. Tyrosylprotein sulfotransferase isoform 2 appeared to recognize the C4 peptide in a deep cleft by using a short parallel β-sheet type interaction, and the bound C4P5Y3 forms an L-shaped structure. Surprisingly, the mode of substrate peptide recognition observed in the tyrosylprotein sulfotransferase isoform 2 structure resembles that observed for the receptor type tyrosine kinases.

PubMed: 23481380
DOI: 10.1038/ncomms2593

Experimental method

X-RAY DIFFRACTION (3.5 Å)