3AP1

Crystal structure of human tyrosylprotein sulfotransferase-2 complexed with PAP and C4 peptide

3AP1 の概要

• エントリーDOI: 10.2210/pdb3ap1/pdb
• 関連するPDBエントリー: 3AP2 3AP3
• 分子名称: Protein-tyrosine sulfotransferase 2, C4 peptide, ADENOSINE-3'-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: sulfotransferase fold, transferase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity): O60704
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 78850.09

構造登録者

Teramoto, T.,Fujikawa, Y.,Kawaguchi, Y.,Kurogi, K.,Soejima, M.,Adachi, R.,Nakanishi, Y.,Mishiro-Sato, E.,Liu, M.-C.,Sakakibara, Y.,Suiko, M.,Kimura, M.,Kakuta, Y. (登録日: 2010-10-09, 公開日: 2011-10-26, 最終更新日: 2024-10-16)

主引用文献

Teramoto, T.,Fujikawa, Y.,Kawaguchi, Y.,Kurogi, K.,Soejima, M.,Adachi, R.,Nakanishi, Y.,Mishiro-Sato, E.,Liu, M.C.,Sakakibara, Y.,Suiko, M.,Kimura, M.,Kakuta, Y.
Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction.
Nat Commun, 4:1572-1572, 2013

PubMed Abstract: Post-translational protein modification by tyrosine sulfation has an important role in extracellular protein-protein interactions. The protein tyrosine sulfation reaction is catalysed by the Golgi enzyme called the tyrosylprotein sulfotransferase. To date, no crystal structure is available for tyrosylprotein sulfotransferase. Detailed mechanism of protein tyrosine sulfation reaction has thus remained unclear. Here we present the first crystal structure of the human tyrosylprotein sulfotransferase isoform 2 complexed with a substrate peptide (C4P5Y3) derived from complement C4 and 3'-phosphoadenosine-5'-phosphate at 1.9 Å resolution. Structural and complementary mutational analyses revealed the molecular basis for catalysis being an SN2-like in-line displacement mechanism. Tyrosylprotein sulfotransferase isoform 2 appeared to recognize the C4 peptide in a deep cleft by using a short parallel β-sheet type interaction, and the bound C4P5Y3 forms an L-shaped structure. Surprisingly, the mode of substrate peptide recognition observed in the tyrosylprotein sulfotransferase isoform 2 structure resembles that observed for the receptor type tyrosine kinases.

PubMed: 23481380
DOI: 10.1038/ncomms2593

実験手法

X-RAY DIFFRACTION (1.9 Å)